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Phosphorescent Nanoparticles

protected dyes with long decay times ideal for lifetime

In addition to the fluorescent PD- and PAN-Chromeo™ Nanoparticles, Active Motif Chromeon has developed a unique line of Phosphorescent PD- and PAN-Nanoparticles. The structure of these nanoparticles provides excellent protection of the dyes against quenchers like oxygen, which can result in low signal intensity if allowed to interact with the dyes. In addition, the excellent shielding of the Phosphorescent PD- and PAN-Nanoparticles result in well-defined luminescent decay times that are in the µs range. This enables them to be used for gated measurements, such as lifetime, which helps exclude any background fluorescence and enhance signal intensity. A variety of dyes and surface chemistries have been combined with the PD and PAN Nanoparticles, providing a multitude of choices in regards to the absorbance, emission, decay time and conjugation properties of the particle for your experiment.

Why Use Phosphorescent Nanoparticles?

• Small size (< 100 nm)
• Large Stokes shifts
• Photostable
• Low oxygen permeability
• Defined decay times

Phosphorescent PAN-Nanoparticles

Phosphorescent PAN-Nanoparticles are highly luminescent; they are much brighter than any other nanoparticles excitable at 470 nm and display a large Stokes shift of approximately 150 nm. Due to their thin wall, PAN-based nanoparticles are ideally suited for FRET applications. Two different ruthenium dyes are available as PAN-Nanoparticles, so you can choose the decay time best suited for your experiment (5.6 or 1.5 µs). For your convenience, the dyes are provided either carboxylated or with surface modifications (proteinic- or tensidic-blocked surfaces) in case carboxy groups interfere with your experiments.

Phosphorescent PD-Nanoparticles

Phosphorescent PD-Nanoparticles are highly impermeable to oxygen. This makes it possible to incorporate phosphorescent dyes that would be quenched by oxygen if used in other types of nanoparticles. Unlike polystyrene-based nanoparticles with porphins, neither the luminescence intensity nor the decay time of PD-Nanoparticles depends on the oxygen concentration of the sample. Five different dyes are available as PD-Nanoparticles, so there is a wide choice of absorption, emission and decay times. For your convenience, all of these dyes are provided either carboxylated or with surface modifications (proteinic- or tensidic-blocked surfaces) in case carboxy groups interfere with your experiments.

Figure 1: Transmission electron micrographs of PD-Ru1-carboxylated nanoparticles.

TEM was performed at the University of Regensburg, Centre for Electron microscopy, Faculty of Natural Sciences III (Biology and preclinical medicine) PD Dr. Reinhard Rachel.

Contents & Storage

All nanoparticles are supplied as a 0.5% (w/w) aqueous suspension in buffer (10 mM MES, pH 7) and are guaranteed stable for 6 months when stored at 4°C.