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Chromeo™ Fluorescent Secondary Antibodies

anti-rabbit & anti-mouse secondaries for enhanced fluorescent detection

Active Motif Chromeon's fluorescent secondary conjugates offer you an improved method for detection of your primary antibodies. These secondary antibodies are conjugated to the Chromeo™ line of fluorescent dyes, which provide you with unmatched fluorescent detection for all your imaging needs. Currently, mouse and rabbit secondary antibodies conjugated to 488, 494, 546 and 642 nm dyes are available.

Chromeo™ labeling advantages

• Unrivaled fluorescent intensity
• Low background
• Limited photobleaching

Figure 1: Tubulin staining with Chromeo 488 secondary antibody.

U2OS cells stained with propidium iodide were incubated with rabbit anti-tubulin antibody. Chromeo 488 Goat anti-Rabbit IgG secondary antibody was then used at 1:1000.

Figure 2: EGFR staining with Chromeo 546 secondary antibody.

The Chromeo 546 Goat anti-Mouse IgG secondary antibody was used to stain EGFR in an urothel cell line. This data was generously provided by Dr. Brockhoff, Institut for Pathology, University Regensburg, Germany.

Contents & Storage

1 mg of antibody at a concentration of 2 mg/ml. For short-term storage, the conjugated antibody should be stored at 4°C protected from light. For longer-term storage, aliquot the antibody and store at -20°C. Avoid subjecting the antibody to repeated freeze-thaw cycles. This product is guaranteed for 6 months from the date of arrival.