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Active Motif Chromeon Fluorescent Dyes

  Lamin primary antibody is
  detected by Chromeo 488
  Goat anti-Mouse IgG
  in HeLa cells.

Chromeo™ Dyes & Fluorescent Secondary Antibodies

Active Motif Chromeon offers a line of novel fluorescent chromophores, the Chromeo Dyes, which are available as reactive labels and conjugates, as well as ready-to-use anti-rabbit and anti-mouse secondary IgGs. These innovative reagents have been optimized for use in a number of different applications, including protein and DNA labeling. The range of Chromeo Dyes matches the most common excitation sources. To find out more, click here.

 Blue when unbound,
 Py-Dyes color shift
 after conjugation.

Py-Dyes

Py-Dyes are a class of amine-reactive dyes that possess unique physical and spectral properties that make them ideal for use in general-purpose protein labeling. Naturally amine reactive, the unbound form of a Py-Dye has a quantum yield of nearly zero. However, upon conjugation the dye short wave shifts and acquires a high quantum yield. The result is a family of dyes that do not require complicated conjugation chemistry, and that provide high-intensity signals with almost no background interference. Thus, Py-Dye members are used in both the ProStain™ Protein Quantification Kit and the CE Dye Kits for capillary electrophoresis. To find out more about Py-Dyes, click here.

  RuLabel structure.

RuLabels

In addition to intensity-based measurements, fluorescent signals can be measured using parameters such as lifetime or polarization. These methods overcome problems associated with auto-fluorescence and background fluorescence because these signals decay rapidly, and because polarized signals are highly specific to the fluorophore being measured. Sensitivity is enhanced because fluorescent signals are read over longer, time-gated periods that amplify signal intensity. Active Motif Chromeon offers a line of RuLabels that are ready for conjugation to biomolecules. These dyes emit polarized light with a long decay time between 400 ns and 5 µs, making them ideal for use in a wide variety of methods. To find out more, click here.

  Phospho-histone H3 (Ser 28)
  detected by Chromeo 488
  Goat anti-Mouse IgG (green)
  in HeLa cells co-labeled with
  propidium iodide (red).

Custom Antibody & Protein Labeling Service

If you don't have a fluorescent conjugate of your antibody or protein of interest, try our custom labeling service. Active Motif Chromeon can conjugate any of its Chromeo Dyes to your antibody or protein, so you can concentrate on your research. Use our expertise in dyes & conjugation to ensure that you get optimal fluorescent conjugates. To find out more about our services, click here.