Your Source for Unique
Fluorescence Research Tools

Over the last 20 years, fluorescence has become increasingly important in biological
and biomedical research. The sensitivity, reproducibility and ability to quantify and to
monitor events in living cells, combined with the increasing number of user-friendly
instruments, have made fluorescence detection valuable for nearly all molecular and
cell biology techniques.

At Active Motif Chromeon, we develop innovative fluorescence-based research tools
that help researchers to improve the detection of molecules in fluorescence microscopy,
flow cytometry or assays that use spectrofluorometers or microplate readers for detection.
Our goal is to provide our customers with high-quality products complemented by superior service & support.

New & Notable Products

• Streptavidin conjugated SiFluor™ Nanoparticles

  To increase intensity in fluorescent assays, Active Motif Chromeon is now offering fluorescent Silica nanoparticles that have been functionalized with Streptavidin molecules. These novel nanobeads have been successfully tested in ELISA-type assays and in immunohistochemistry applications where they exhibit enhanced fluorescent intensity and detection sensitivity. More >

• New Secondary Antibodies for Super-resolution Microscopy

  Active Motif Chromeo continues to expand its portfolio of products suitable for Super-resolution Microscopy. Our new ATTO 532 (GSD) and Rhodamine 6G (GSD) secondary antibody conjugates have been developed for use in GSDIM (Ground State Depletion with Individual Molecule return) microscopy on the Leica GSD microscope. More >

• Chromeo™ Tools for GSDIM Microscopy

  GSDIM (Ground State Depletion with Individual Molecule return) microscopy is another super-resolution microscopy technology that has recently been launched by Leica Microsystems. It enables researcher to image biological samples with a resolution as low as 20 nm. Leica Microsystems recommends our Chromeo™ 488, Chromeo™ 505 and Chromeo™ 546 fluorescent dyes and secondary antibody conjugates for use with their instrument.

• Azide and Alkyne Derivatives of Chromeo™ Dyes for Specific Labeling

  Click-chemistry, the chemical reaction between alkyne and azide that results in a stable covalent bond, is becoming widely used for labeling and for detection of biomolecules, as well as in general synthesis approaches like the production of large multimeric molecules. Because neither reaction partner occurs in nature, this highly specific reaction is suitable for bio-orthogonal labeling of sugar molecules, proteins or DNA in the complex environment of a living cell. Active Motif Chromeon now offers fluorescent Chromeo™ Dyes in azide and alkyne formats. More >

• Fluorescent Nanoparticles Increase Sensitivity

  When trying to detect targets that occur in low concentrations, adding more fluorescent dye does not always increase the signal intensity due to the effects of quenching. A better way to increase sensitivity can be Fluorescent Nanoparticles. Each nanoparticle contains hundreds of fluorophores at the dye’s optimal dye/nanoparticle ratio, which has been determined to provide the brightest possible signal. A variety of dyes and attachment chemistries are offered. More >

• Chameleon Dyes Provide Background-free Fluorescent Labeling

  Because Chromeo™ Py-Dyes do not become fluorescent until after reacting with an amine group, there is no background from any unbound dye following the simple one-step, room-temperature incubation. As there is no need for purification before use, pyrylium-based Py-Dyes are ideal for “no-wash” applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies. More >

• Long Decay RuLabels for Lifetime and Polarization-based Assays

  Chromeo™ RuLabels are phosphorescent ruthenium probes that emit strongly polarized light with long decay times (between 400 ns and 5 µs) and large Stokes shifts, making possible a wide range of detection methods including intensity, decay time (lifetime) and polarization-based measurements. More >

• Measure Oxygen Concentration Using Fluorescence Quenching

  Active Motif Chromeon offers a variety of ruthenium probes whose fluorescence is dynamically quenched by oxygen. Therefore, these “oxygen-sensing” probes are ideal for measuring the concentration of oxygen in a wide variety of organic and aqueous solvents. The probes have decay times in the microsecond range (2-4 µs), so can be used to measure oxygen by either intensity or decay time. More >

• Monitor Oxidative Stress Pathways with the EuTc-H₂O₂ Assay

  Europium Tetracycline (EuTc) becomes strongly fluorescent only after it combines with hydrogen peroxide (H₂O₂). Because free H₂O₂ is produced by a number of enzymes involved in oxidative stress-related states, the EuTc-H₂O₂ Assay is a sensitive, easy-to-use method to study the activity of catalases, peroxidases and other enzymes (and their inhibitors) that produce and eliminate hydrogen peroxide. More >