Try Chameleon Dyes
Chromeo™ Py-Dyes change their color and become fluorescent only after binding to their targets, eliminating background fluorescence from any unbound dye. This makes them easier to conjugate and use in your assays.
Learn MoreChromeo™ Py-Dyes change their color and become fluorescent only after binding to their targets, eliminating background fluorescence from any unbound dye. This makes them easier to conjugate and use in your assays.
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Click Chemistry is an extremely specific labeling
method because it uses 2 reaction partners that
do not occur in nature. The Azide and Alkyne
derivatives of Chromeo™ Dyes make it easy
to take advantage of this powerful method.
STimulated Emission Depletion (STED) can
provide up to 12-fold greater resolution than
confocal microscopy. Leica recommends our
STED sample preparation kit and fluorescent
secondaries for use with its STED instruments.
All of our Fluorescent Secondary Antibodies are
prepared using an optimized conjugation protocol.
This makes their fluorescent signals brighter,
while minimizing background fluorescence.
Techniques like STED and GSDIM microscopy “break” the diffraction barrier, enabling image resolutions as low as 20 nm. Leica Microsystems has tested and recommends many of Active Motif Chromeon’s fluorescent dyes and secondary antibody conjugates for use with its super-resolution instruments.
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Over the last 20 years, fluorescence has become increasingly important in biological
and biomedical research. The sensitivity, reproducibility and ability to quantify and to
monitor events in living cells, combined with the increasing number of user-friendly
instruments, have made fluorescence detection valuable for nearly all molecular and
cell biology techniques.
At Active Motif Chromeon, we develop innovative fluorescence-based research tools
that help researchers to improve the detection of molecules in fluorescence microscopy,
flow cytometry or assays that use spectrofluorometers or microplate readers for detection.
Our goal is to provide our customers with high-quality products complemented by superior service & support.
To increase intensity in fluorescent assays, Active Motif Chromeon is now offering fluorescent Silica nanoparticles that have been functionalized with Streptavidin molecules. These novel nanobeads have been successfully tested in ELISA-type assays and in immunohistochemistry applications where they exhibit enhanced fluorescent intensity and detection sensitivity. More >
Active Motif Chromeo continues to expand its portfolio of products suitable for Super-resolution Microscopy. Our new ATTO 532 (GSD) and Rhodamine 6G (GSD) secondary antibody conjugates have been developed for use in GSDIM (Ground State Depletion with Individual Molecule return) microscopy on the Leica GSD microscope. More >
GSDIM (Ground State Depletion with Individual Molecule return) microscopy is another super-resolution microscopy technology that has recently been launched by Leica Microsystems. It enables researcher to image biological samples with a resolution as low as 20 nm. Leica Microsystems recommends our Chromeo™ 488, Chromeo™ 505 and Chromeo™ 546 fluorescent dyes and secondary antibody conjugates for use with their instrument.
Click-chemistry, the chemical reaction between alkyne and azide that results in a stable covalent bond, is becoming widely used for labeling and for detection of biomolecules, as well as in general synthesis approaches like the production of large multimeric molecules. Because neither reaction partner occurs in nature, this highly specific reaction is suitable for bio-orthogonal labeling of sugar molecules, proteins or DNA in the complex environment of a living cell. Active Motif Chromeon now offers fluorescent Chromeo™ Dyes in azide and alkyne formats. More >
When trying to detect targets that occur in low concentrations, adding more fluorescent dye does not always increase the signal intensity due to the effects of quenching. A better way to increase sensitivity can be Fluorescent Nanoparticles. Each nanoparticle contains hundreds of fluorophores at the dye’s optimal dye/nanoparticle ratio, which has been determined to provide the brightest possible signal. A variety of dyes and attachment chemistries are offered. More >
Because Chromeo™ Py-Dyes do not become fluorescent until after reacting with an amine group, there is no background from any unbound dye following the simple one-step, room-temperature incubation. As there is no need for purification before use, pyrylium-based Py-Dyes are ideal for “no-wash” applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies. More >
Chromeo™ RuLabels are phosphorescent ruthenium probes that emit strongly polarized light with long decay times (between 400 ns and 5 µs) and large Stokes shifts, making possible a wide range of detection methods including intensity, decay time (lifetime) and polarization-based measurements. More >
Active Motif Chromeon offers a variety of ruthenium probes whose fluorescence is dynamically quenched by oxygen. Therefore, these “oxygen-sensing” probes are ideal for measuring the concentration of oxygen in a wide variety of organic and aqueous solvents. The probes have decay times in the microsecond range (2-4 µs), so can be used to measure oxygen by either intensity or decay time. More >
Europium Tetracycline (EuTc) becomes strongly fluorescent only after it combines with hydrogen peroxide (H2O2). Because free H2O2 is produced by a number of enzymes involved in oxidative stress-related states, the EuTc-H2O2 Assay is a sensitive, easy-to-use method to study the activity of catalases, peroxidases and other enzymes (and their inhibitors) that produce and eliminate hydrogen peroxide. More >
Download Active Motif Chromeon manuals, product brochures and Technical Data Sheets, or request more information.
Find papers relevant to your research that cite the use of our products. More >
View our latest posts of notable
papers on fluorescence.
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Get our manuals and data sheets,
or request more information.
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